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NONO / p54nrb Rabbit mAb#59836

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Product Detail

Product NameNONO / p54nrb Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityNONO / p54nrb Antibody detects endogenous levels of total NONO / p54nrb

Immunogen DescA synthesized peptide derived from human NONO / p54nrb

Other NamesP54; NMT55; NRB54; P54NRB;

Accession NoUniprot:Q15233

Uniprot Q15233

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:100~1:500
Western blot analysis of NONO / p54nrb expression in (1) MOLT4 cell lysate; (2) NIH/3T3 cell lysate; (3) PC12 cell lysate.
DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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