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Location： Home > Recombinant Rabbit Monoclonal Antibodies > CBFb Rabbit mAb

CBFb Rabbit mAb#59850

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Product Detail

Product NameCBFb Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificityCBFb Antibody detects endogenous levels of total CBFb

Immunogen DescA synthesized peptide derived from human CBFb

Other NamesCBFB; CBFbeta; PEA2; PEA2 beta; PEA2beta; PEBP2 beta; PEBP2B;

Accession NoUniprot:Q13951

Uniprot Q13951

Calculated MWPredicted band size: 22 kDa

Sdspage MWObserved band size: 22 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: CBFb Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : K562 whole cell lysates
Lane 3 : Raw264.7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 8 seconds
All lanes:CBFb Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :CBFb Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human spleen tissue stained for CBFb using 59850 at 1/100 dilution in immunohistochemical analysis.
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.

If you have published an article using product 59850, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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