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Retinoid X Receptor alpha Rabbit mAb#59868

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Product Detail

Product NameRetinoid X Receptor alpha Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityRetinoid X Receptor alpha Antibody detects endogenous levels of total Retinoid X Receptor alpha

Immunogen DescA synthesized peptide derived from human Retinoid X Receptor alpha

Other NamesNR2B1; RXR alpha1; Rxra; RXRalpha1;

Accession NoUniprot:P19793

Uniprot P19793

Calculated MWPredicted band size: 51 kDa

Sdspage MWObserved band size: 51 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Retinoid X Receptor alpha Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : K562 whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 8 seconds
All lanes:Retinoid X Receptor alpha Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :Retinoid X Receptor alpha Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for Retinoid X Receptor alpha using 59868 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Retinoid X Receptor alpha antibody (59868)

ICC/IF staining of Retinoid X Receptor alpha in MCF-7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 59868 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes.

If you have published an article using product 59868, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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