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Location： Home > Recombinant Rabbit Monoclonal Antibodies > NEK2 Rabbit mAb

NEK2 Rabbit mAb#59950

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Product Detail

Product NameNEK2 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman

SpecificityNEK2 Antibody detects endogenous levels of total NEK2

Immunogen DescA synthesized peptide derived from human NEK2

Other NamesHsPK21; NEK2; NEK2A; NLK1;

Accession NoUniprot:P51955

Uniprot P51955

Calculated MWPredicted band size: 52 kDa

Sdspage MWObserved band size: 44,48 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes: NEK2 Rabbit mAb at 1/1k dilution

Lane 1 : 293 whole cell lysates
Lane 2 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 44,48 kDa

Exposure time: 8 seconds
All lanes:NEK2 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :NEK2 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore.

If you have published an article using product 59950, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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