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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Legumain Rabbit mAb

Legumain Rabbit mAb#59991

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Product Detail

Product NameLegumain Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificityLegumain Antibody detects endogenous levels of total Legumain

Immunogen DescA synthesized peptide derived from human Legumain

Other NamesAEP; cysteine 1; Legumain; LGMN; LGMN1; PRSC1;

Accession NoUniprot:Q99538

Uniprot Q99538

Calculated MWPredicted band size: 49 kDa

Sdspage MW36,49,57 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: MTH1 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 49 kDa
Observed band size: 36,49,57 kDa

Exposure time: 4 seconds
All lanes: MTH1 Rabbit mAb at 1/1k dilution

Lane 1 : Mouse spleen lysates
Lane 2 : Rat kidney lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 49 kDa
Observed band size: 36,49,57 kDa

Exposure time: 7 seconds
All lanes:Legumain Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :Legumain Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Legumain using 59991 at 1/100 dilution in immunohistochemical analysis.
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

If you have published an article using product 59991, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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