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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Histone H3 (acetyl K18) Rabbit mAb

Histone H3 (acetyl K18) Rabbit mAb#60055

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Product Detail

Product NameHistone H3 (acetyl K18) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityHistone H3 (acetyl K18) Antibody detects endogenous levels of total Histone H3 (acetyl K18)

Immunogen DescA synthesized peptide derived from Histone H3 (acetyl K18)

Other NamesHistone H3.1, Histone H3, HIST1H3A;

Accession NoUniprot:P68431

Uniprot P68431

Calculated MWPredicted band size: 15 kDa

Sdspage MWObserved band size: 17 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Histone H3 (acetyl K18) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : Mouse lung lysates
Lane 4 : Rat lung lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 17 kDa

Exposure time: 8 seconds
All lanes:Histone H3 (acetyl K18) Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :Histone H3 (acetyl K18) Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Histone H3 (acetyl K18) using 60055 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Histone H3 (acetyl K18) antibody (60055)

ICC/IF staining of Histone H3 (acetyl K18) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 60055 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Belongs to the histone H3 family. Play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

If you have published an article using product 60055, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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