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RASA1 Rabbit mAb#60072

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Product Detail

Product NameRASA1 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Rat

SpecificityRASA1 Antibody detects endogenous levels of total RASA1

Immunogen DescA synthesized peptide derived from human RASA1

Other NamesCMAVM; GAP; p120GAP; p120RASGAP; PKWS; RASA; RASA1; RasGAP;

Accession NoUniprot:P20936

Uniprot P20936

Calculated MWPredicted band size: 116 kDa

Sdspage MWObserved band size: 140 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: RASA1 Rabbit mAb at 1/1k dilution

Lane 1 : Rat brain cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 116 kDa
Observed band size: 140 kDa

Exposure time: 3 seconds
All lanes:RASA1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 :RASA1 Rabbit mAb knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for RASA1 using 60072 at 1/100 dilution in immunohistochemical analysis.
Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.

If you have published an article using product 60072, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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