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Location： Home > Recombinant Rabbit Monoclonal Antibodies > MUTA Rabbit mAb

MUTA Rabbit mAb#60075

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Product Detail

Product NameMUTA Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificityMUTA Antibody detects endogenous levels of total MUTA

Immunogen DescA synthesized peptide derived from human MUTA

Other NamesMCM; Methylmalonyl CoA isomerase; Mut;

Accession NoUniprot:P22033

Uniprot P22033

Calculated MWPredicted band size: 83 kDa

Sdspage MWObserved band size: 78 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: MUTA Rabbit mAb at 1/1k dilution

Lane 1 : JK whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lane 3 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 83 kDa
Observed band size: 78 kDa

Exposure time: 4 seconds
All lanes: MUTA Rabbit mAb at 1/1k dilution

Lane 1 : Mouse heart lysates
Lane 2 : Mouse liver lysates
Lane 3 : Rat heart lysates
Lane 4 : Rat liver lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 83 kDa
Observed band size: 78 kDa

Exposure time: 6 seconds
All lanes:MUTA Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :MUTA Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human cholangiocarcinoma tissue stained for MUTA using 60075 at 1/100 dilution in immunohistochemical analysis.
Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.

If you have published an article using product 60075, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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