Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Recombinant Rabbit Monoclonal Antibodies > ZHX2 Rabbit mAb

ZHX2 Rabbit mAb#60108

Availability: Yes
Total Amount:$
Product Detail

Product NameZHX2 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityZHX2 Antibody detects endogenous levels of total ZHX2

Immunogen DescA synthesized peptide derived from human ZHX2

Other NamesAFP regulator 1; AFR1; Alpha fetoprotein regulator 1; RAF; Regulator of AFP; Transcription factor ZHX2;ZHX2; Zinc finger and homeodomain protein 2; Zinc fingers and homeoboxes 2; Zinc fingers and homeoboxes protein 2;

Accession NoUniprot:Q9Y6X8

Uniprot Q9Y6X8

Calculated MWPredicted band size: 92 kDa

Sdspage MWObserved band size: 110 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes: ZHX2 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lane 3 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 92 kDa
Observed band size: 110 kDa

Exposure time: 7 seconds
All lanes:ZHX2 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :ZHX2 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence ZHX2 antibody (60108)

ICC/IF staining of ZHX2 in MCF-7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 60108 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Acts as a transcriptional repressor.

If you have published an article using product 60108, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;