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Location： Home > Recombinant Rabbit Monoclonal Antibodies > ENDOGL1 / ENGL Rabbit mAb

ENDOGL1 / ENGL Rabbit mAb#60109

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Product Detail

Product NameENDOGL1 / ENGL Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman

SpecificityENDOGL1 / ENGL Antibody detects endogenous levels of total ENDOGL1 / ENGL

Immunogen DescA synthesized peptide derived from human ENDOGL1 / ENGL

Other NamesEndo G like 1; ENDOGL1; ENDOGL2; Endonuclease G like 1; Endonuclease G like 2; ENGL; ENGLA; ENGLB; Exog; Nuclease EXOG;

Accession NoUniprot:Q9Y2C4

Uniprot Q9Y2C4

Calculated MWPredicted band size: 41 kDa

Sdspage MWObserved band size: 41 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: ENDOGL1 / ENGL Rabbit mAb at 1/1k dilution

Lane 1 : HepG2 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 41 kDa
Observed band size: 41 kDa

Exposure time: 3 seconds
All lanes:ENDOGL1 / ENGL Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :ENDOGL1 / ENGL Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human heart tissue stained for ENDOGL1 / ENGL using 60109 at 1/100 dilution in immunohistochemical analysis.
Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single stranded DNA and 5'-3' exonuclease activity.

If you have published an article using product 60109, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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