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Cip4 Rabbit mAb#60117

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Product Detail

Product NameCip4 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse

SpecificityCip4 Antibody detects endogenous levels of total Cip4

Immunogen DescA synthesized peptide derived from human Cip4

Other NamesCdc42 interacting protein 4; CIP4; DCIP4; hSTP; STOT; STP; Thyroid receptor interacting protein 10; trip10;

Accession NoUniprot:Q15642

Uniprot Q15642

Calculated MWPredicted band size: 68 kDa

Sdspage MWObserved band size: 75 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: Cip4 Rabbit mAb at 1/1k dilution

Lane 1 : HepG2 whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 75 kDa

Exposure time: 8 seconds
All lanes:Cip4 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 :Cip4 Rabbit mAb knockdown HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Cip4 using 60117 at 1/100 dilution in immunohistochemical analysis.
Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity).

If you have published an article using product 60117, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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