Product Detail
Product NameC3orf38 Rabbit mAb
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
PurificationAffinity-chromatography
ApplicationsWB ICC/IF
Species ReactivityHuman
SpecificityC3orf38 Antibody detects endogenous levels of total C3orf38
Immunogen DescA synthesized peptide derived from human C3orf38
Other NamesC3orf38;
Accession NoUniprot:Q5JPI3
Uniprot
Q5JPI3
Calculated MWPredicted band size: 38 kDa
Sdspage MWObserved band size: 38 kDa
FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.
Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes: C3orf38 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates Lane 2 : JK whole cell lysates Lane 3 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 7 seconds
All lanes:C3orf38 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 :C3orf38 Rabbit mAb knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence C3orf38 antibody (60155) ICC/IF staining of C3orf38 in U-2 OS cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 60155 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
May be involved in apoptosis regulation.
If you have published an article using product 60155, please notify us so that we can cite your literature.