Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Recombinant Rabbit Monoclonal Antibodies > C3orf38 Rabbit mAb

C3orf38 Rabbit mAb#60155

Availability: Yes
Total Amount:$
Product Detail

Product NameC3orf38 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB ICC/IF

Species ReactivityHuman

SpecificityC3orf38 Antibody detects endogenous levels of total C3orf38

Immunogen DescA synthesized peptide derived from human C3orf38

Other NamesC3orf38;

Accession NoUniprot:Q5JPI3

Uniprot Q5JPI3

Calculated MWPredicted band size: 38 kDa

Sdspage MWObserved band size: 38 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes: C3orf38 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 38 kDa
Observed band size: 38 kDa

Exposure time: 7 seconds
All lanes:C3orf38 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :C3orf38 Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence C3orf38 antibody (60155)

ICC/IF staining of C3orf38 in U-2 OS cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 60155 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
May be involved in apoptosis regulation.

If you have published an article using product 60155, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;