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TSFM Rabbit mAb#60156

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Product Detail

Product NameTSFM Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityTSFM Antibody detects endogenous levels of total TSFM

Immunogen DescA synthesized peptide derived from human TSFM

Other NamesCOXPD3; EFTS; EFTSMT; TSFM; TSMT;

Accession NoUniprot:P43897

Uniprot P43897

Calculated MWPredicted band size: 35 kDa

Sdspage MWObserved band size: 35 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: TSFM Rabbit mAb at 1/1k dilution

Lane 1 : HepG2 whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : Mouse liver lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 5 seconds
All lanes:TSFM Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :TSFM Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for TSFM using 60156 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for TSFM using 60156 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence TSFM antibody (60156)

ICC/IF staining of TSFM in HuT 102 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 60156 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.

If you have published an article using product 60156, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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