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PPM1G Rabbit mAb#60173

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Product Detail

Product NamePPM1G Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificityPPM1G Antibody detects endogenous levels of total PPM1G

Immunogen DescA synthesized peptide derived from human PPM1G

Other NamesPP2C; PP2C gamma; PP2CG; PPM1G; PPP2CG; Protein phosphatase 1C; Protein phosphatase 1G;

Accession NoUniprot:O15355

Uniprot O15355

Calculated MWPredicted band size: 59 kDa

Sdspage MWObserved band size: 73 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: PPM1G Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 59 kDa
Observed band size: 73 kDa

Exposure time: 5 seconds
All lanes:PPM1G Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 :PPM1G Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PPM1G using 60173 at 1/100 dilution in immunohistochemical analysis.
Belongs to the PP2C family.

If you have published an article using product 60173, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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