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Location： Home > Recombinant Rabbit Monoclonal Antibodies > AER61 Rabbit mAb

AER61 Rabbit mAb#60192

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Product Detail

Product NameAER61 Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Mouse Rat

SpecificityAER61 Antibody detects endogenous levels of total AER61

Immunogen DescA synthesized peptide derived from human AER61

Other NamesAOS4; C3orf64; EOGT; EOGT1;

Accession NoUniprot:Q5NDL2

Uniprot Q5NDL2

Calculated MWPredicted band size: 62 kDa

Sdspage MWObserved band size: 55 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes: AER61 Rabbit mAb at 1/1k dilution

Lane 1 : Caco 2 whole cell lysates
Lane 2 : Mouse kidney lysates
Lane 3 : Rat kidney lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 55 kDa

Exposure time: 9 seconds
All lanes:AER61 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 :AER61 Rabbit mAb knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.
Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.

If you have published an article using product 60192, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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