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GNAQ Rabbit mAb#60244

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Product Detail

Product NameGNAQ Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificityGNAQ Antibody detects endogenous levels of total GNAQ

Immunogen DescA synthesized peptide derived from human GNAQ

Other NamesCMC1; G alpha Q; G protein alpha Q ; GAQ; GNAQ;

Accession NoUniprot:P50148

Uniprot P50148

Calculated MWPredicted band size: 42 kDa

Sdspage MWObserved band size: 42 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: GNAQ Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : 293 whole cell lysates
Lane 3 : K562 whole cell lysates
Lane 4 : 3T3 whole cell lysates
Lane 5 : Mouse brain lysates
Lane 6 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 5 seconds
All lanes:GNAQ Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : GNAQ Rabbit mAb knockdown HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast tissue stained for GNAQ using 60244 at 1/100 dilution in immunohistochemical analysis.
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

If you have published an article using product 60244, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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