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Location： Home > Recombinant Rabbit Monoclonal Antibodies > PKC delta + PKC theta Rabbit mAb

PKC delta + PKC theta Rabbit mAb#60289

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Product Detail

Product NamePKC delta + PKC theta Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthesized peptide derived from human PKC delta + PKC theta

ConjugateUnconjugated

Uniprot Q05655/Q04759/P28867/

Calculated MWPredicted band size: 78 kDa

Sdspage MWObserved band size: 78 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: PKC delta + PKC theta Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lane 4 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 78 kDa
Observed band size: 78 kDa

Exposure time: 5 seconds
All lanes: PKC delta + PKC theta Rabbit mAb at 1/1k dilution

Lane 1 : Mouse heart lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 78 kDa
Observed band size: 78 kDa

Exposure time: 4 seconds
All lanes:PKC delta + PKC theta Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : PKC delta + PKC theta Rabbit mAb knockdown A549 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for PKC delta + PKC theta using 60289 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence PKC delta + PKC theta antibody (60289)

ICC/IF staining of PKC delta + PKC theta in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 60289 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

 Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses (PubMed:21810427, PubMed:21406692).

If you have published an article using product 60289, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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