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NFAT1(Phospho-Ser53) Antibody#62089

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Product Detail

Product NameNFAT1(Phospho-Ser53) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

ApplicationsWB

Species ReactivityHuman

SpecificityThe antibody detects endogenous level of NFAT1 only when phosphorylated at serine 53.

Immunogen TypePeptide

Immunogen DescA synthesized peptide derived from human NFAT1 around the phosphorylation site of Ser53.

Target NameNFATC2

ConjugateUnconjugated

Uniprot Q13469

Gene ID 4773

Calculated MW100kDa

Concentration1mg/ml

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details
WB 1:500-1:2000
All lanes : NFAT1(Phospho-Ser53) Antibody at 1/1K dilution
Lane 1 : treated with blocking peptide
Lane 2 : Hela cells(heat shock treatment) lysates
Lysates/proteins at 20 ug per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 100-150 kDa

Exposure time: 4 seconds
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF (PubMed:15790681). Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway (PubMed:21871017). Is involved in the negative regulation of chondrogenesis (PubMed:35789258). Recruited by AKAP5 to ORAI1 pore-forming subunit of CRAC channels in Ca2+ signaling microdomains where store-operated Ca2+ influx is coupled to calmodulin and calcineurin signaling and activation of NFAT-dependent transcriptional responses.

If you have published an article using product 62089, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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