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Beclin 1 (Phospho-Ser295) Antibody#62092

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Product Detail

Product NameBeclin 1 (Phospho-Ser295) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

ApplicationsIHC,IF/ICC

Species ReactivityHuman,Mouse,Rat

SpecificityThe antibody detects endogenous level of Beclin 1 only when phosphorylated at Ser295

Immunogen TypePeptide

Immunogen DescPeptide sequence around phosphorylation site of Ser295 (L-P-S(p)-V-P) derived from HumanBeclin 1.

Target NameBeclin 1

ConjugateUnconjugated.

Uniprot Q14457

Gene ID 8678

Calculated MW52kD(Calculated)

Concentration1mg/ml

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details

IHC 1:50-1:200, IF/ICC 1:100-1:500

Formalin-fixed,paraffin-embedded Human esophageal cancer and adjacent normal tissues stained for Phospho-Ser295 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed,paraffin-embedded Human kidney cancer and adjacent normal tissues stained for Phospho-Ser295  at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed,paraffin-embedded Human kidney cancer tissues stained for Phospho-Ser295 at 1/100 dilution in immunohistochemical analysis
Immunocytochemistry/ Immunofluorescence –Beclin 1 (Phospho-Ser295) Antibody

staining Beclin 1 (Phospho-Ser295) Antibody in A549 cells. The cells were fixed with 4% paraformaldehyde permeabilized with 0.1% Triton X-100.
The cells were incubated overnight at 4°C with Antibody at a working dilution of 1/100 and Mouse monoclonal to alpha Tubulin - Loading Control. Cells were incubated with Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red) and Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor®488), pre-adsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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