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Beta Catenin (Phospho-Tyr142) Antibody#62094

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Product Detail

Product NameBeta Catenin (Phospho-Tyr142) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

ApplicationsWB,IF/ICC

Species ReactivityHuman, Mouse, Rat, Monkey

SpecificityThe antibody detects endogenous level of beta Catenin only when phosphorylated at Tyr142

Immunogen TypePeptide

Immunogen DescPeptide sequence around phosphorylation site of Tyr 142(I-N-Y(p)-Q-D) derived from Human beta Catenin.

Target Namebeta Catenin

ConjugateUnconjugated.

Uniprot P35222

Gene ID 1499

Calculated MW92kDa; 85kD(Calculated)

Concentration1mg/ml

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details
WB 1:1000-3000, IF/ICC 1:100-1:500
All lanes : Beta Catenin (Phospho-Tyr142) Antibody at 1/1k dilution

Lane 1 :treated with blocking peptide
Lane 2 :VERO whole cell (serum starvation treatment)lysates
Lysates/proteins at 20 ug per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 85 kDa
Observed band size: 85 kDa

Exposure time: 16 seconds

If you have published an article using product 62094, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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