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High density lipoprotein Antibody#62102

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Product Detail

Product NameHigh density lipoprotein Antibody

Host SpeciesRabbit

ClonalityPolyclonal

Purificationaffinity purified by Protein A

ApplicationsWB;IHC;IF

Species ReactivityHuman; Mouse

SpecificityThe antibody detects endogenous level of total High density lipoprotein protein

Immunogen Deschuman high density lipoprotein purified from plasma

Calculated MW28 kDa

Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details
WB 1:500-2000;IHC 1:100-500; IF 1:100-500
All lanes : High density lipoprotein Antibody at 1/1k dilution

Lane 1 : Mouse Serum
Lysates/proteins at 20 ug per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution

Predicted band size: 28 kDa
Observed band size: 28kDa

Exposure time: 15 seconds
Formalin-fixed, paraffin-embedded Human liver carcinoma stained for High density lipoprotein Antibody was diluted at 1:400 dilution in immunohistochemical analysis.
High-density lipoproteins (HDL) are one of the five major groups of lipoproteins. Lipoproteins are complex particles composed of multiple proteins which transport all fat molecules (lipids) around the body within the water outside cells. They are typically composed of 80-100 proteins per particle (organized by one, two or three ApoA; more as the particles enlarge picking up and carrying more fat molecules) and transporting up to hundreds of fat molecules per particle. Unlike the larger lipoprotein particles which deliver fat molecules to cells, HDL particles remove fat molecules from cells which need to export fat molecules. The fats carried include cholesterol, phospholipids, and triglycerides; amounts of each are quite variable.

If you have published an article using product 62102, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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