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Influenza A Virus Nucleoprotein H1N1 Mouse mAb#62142

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Product Detail

Product NameInfluenza A Virus Nucleoprotein H1N1 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

PurificationThe antibody was affinity purified by Protein A.

ApplicationsWB

Species ReactivityInfluenza A Nucleoprotein

Immunogen TypeProtein

Immunogen DescRecombinant Influenza A virus H1N1(A/Victoria/4897/2022)Nucleoprotein

Target NameInfluenza A Virus Nucleoprotein H1N1

Calculated MW57kDa

Concentration1mg/ml

FormulationLiquid in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

StorageStore at +4˚C for short term. Store at -20˚C for long term. Avoid freeze/thaw cycle.

Application Details
WB 1:500-2000
All lanes : Influenza A Virus Nucleoprotein H1N1 Mouse mAb at 1/1k dilution
Lane 1: rInfluenza A virus H1N1(A/Victoria/4897/2022)Nucleoprotein-His protein proteins at 20ng per lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 10 seconds
The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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