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Location： Home > Polyclonal Antibodies > NPTII/Neomycin Phosphotransferase 2 antibody

NPTII/Neomycin Phosphotransferase 2 antibody#62143

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Product Detail

Product NameNPTII/Neomycin Phosphotransferase 2 antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity purified by Protein A.

ApplicationsWB, IHC-P, IHC-F, IF, ICC/IF, ELISA

Species ReactivityKlebsiella pneumoniae

Immunogen TypePeptide

Immunogen Descpeptide derived from Klebsiella pneumoniae Neomycin Phosphotransferase 2:?101-200/264

Target NameNPTII/Neomycin Phosphotransferase 2

Uniprot P00552

Calculated MW32 kDa

Concentration1mg/ml

FormulationLiquid in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

StorageStore at +4˚C for short term. Store at -20˚C for long term. Avoid freeze/thaw cycle.

Application Details

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin? G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

If you have published an article using product 62143, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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