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FbpB antibody#62145

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Product Detail

Product NameFbpB antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity purified by Protein A.

ApplicationsWB

Immunogen TypePeptide

Immunogen Descpeptide derived from Mycobacterium tuberculosis Ag85B: 231-325/325

Target NameMycobacterium tuberculosis Ag85B

Calculated MW31 kDa

Concentration1mg/ml

FormulationLiquid in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

StorageStore at +4˚C for short term. Store at -20˚C for long term. Avoid freeze/thaw cycle.

Application Details
WB 1:500-2000
All lanes : FbpB antibody at 1/1k dilution
Lane 1: Recombinant M.tuberculosis fbpB protein
Lysates/proteins at 30 ug per lane.
Secondary
All lanes : IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 7 seconds
Antigen 85B is the most abundant protein expressed by Mycobacterium tuberculosis (about one quarter). It is a myocolyc transferase in the myc pathway and catalyses - like Ag85A and Ag85C - the transfer of the fatty acid mycolate from one trehalose monomycolate to another, resulting in trehalose dimycolate and free trehalose and helping build the cell wall.

If you have published an article using product 62145, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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