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GLS1 antibody#62148

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Product Detail

Product NameGLS1 antibody

Host SpeciesRabbit

ClonalityPolyclonal

Purificationaffinity purified by Protein A.

ApplicationsWB, IHC, IF, ELISA

Species ReactivityHuman, Mouse, Rat

SpecificityThis antibody detects endogenous levels of ZN558.

Immunogen DescKLH conjugated synthetic peptide derived from human GLS1: 141-240/669

Target NameGLS1

Uniprot O94925

Gene ID 2744

Calculated MW72 kDa

Concentration1mg/ml

Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details
WB 1:500-2000, IHC-P 1:100-500, IHC-F 1:100-500, IF 1:100-500, ELISA 1:5000-10000
Western blot analysis of lysates using GLS1 antibody at 1/1000 dilution Lane 1: Human U251 cell lysates Lane 2: Human HeLa cell lysates
Immunohistochemical analysis of paraffin-embedded Rat brain with GLS1 Antibody at 1:400.
Immunohistochemical analysis of paraffin-embedded Mouse brain with GLS1 Antibody at 1:400.
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012].

If you have published an article using product 62148, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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