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EGFP-tag Antibody#62163

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Product Detail

Product NameEGFP-tag Antibody

Clone No.MR4543

Host SpeciesMouse

ClonalityMonoclonal

PurificationThe antibody was purified by immunogen affinity chromatography.

ApplicationsWB;IF/IC;IP

Species ReactivitySpecies independent

SpecificityThe antibody detects endogenous level of total EGFP-tag protein.

Immunogen TypeProtein

Immunogen DescRecombinant protein corresponding to EGFP-tag.

ConjugateUnconjugated

FormulationLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details
WB 1:2000 - 1:5000; IF/IC 1:200 - 1:500;IP 1:100 - 1:200
All lanes : EGFP-tag Antibody at 1/2K dilution
Lane 1 : over-expressed EGFP-tagged protein in 293T cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Exposure time: 10 seconds
Immunocytochemistry/ Immunofluorescence EGFP-tag antibody (62163)

ICC/IF staining of EGFP-tag antibody in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 62163 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti mouse, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 62163, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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