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Semaphorin 4B Antibody#62166

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Product Detail

Product NameSemaphorin 4B Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was purified by immunogen affinity chromatography.

ApplicationsWB

Species ReactivityHuman

SpecificityThe antibody detects endogenous level of total Semaphorin 4B protein.

Immunogen TypePeptide

Immunogen DescKLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human Semaphorin 4B. The exact sequence is proprietary.

Target NameSEMA4B

ConjugateUnconjugated

Uniprot Q9NPR2

Gene ID 10509

Calculated MW105kDa

Concentration1mg/ml

FormulationLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details
WB 1:500-1:1000
All lanes : Semaphorin 4B Antibody at 1/1K dilution
Lane 1 : A549 whole cell lysates
Lane 2 : DU145 whole cell lysates
Lysates/proteins at 20 ug per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 105 kDa

Exposure time: 12 seconds

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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