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Location： Home > Polyclonal Antibodies > PI 3 kinase p85/p55 (Phospho Tyr467/199) Antibody

PI 3 kinase p85/p55 (Phospho Tyr467/199) Antibody#62171

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Product Detail

Product NamePI 3 kinase p85/p55 (Phospho Tyr467/199) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB;IHC;IF

Species ReactivityHuman; Mouse; Rat

SpecificityThe antibody detects endogenous level of PI 3 kinase p85/p55 only when phosphorylated at Tyr467/199.

Immunogen TypePeptide

Immunogen DescThe antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma around the phosphorylation site of Tyr467/199

Target NamePIK3R1 ;GRB1 ;Phosphatidylinositol 3-kinase regulatory subunit alpha ;

Uniprot P27986;Q92569;

Gene ID 5295;

Calculated MW55kD;85kDa

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000;

All lanes : PI 3 kinase p85/p55 (Phospho Tyr467/199) Antibody at 1/1k dilution

Lane 1 : HUVEC whole cell lysates
Lane 2 : Hela whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lane 4 : HepG2 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution

Predicted band size: 83,54 kDa
Observed band size: 85,55 kDa

Exposure time: 21 seconds
All lanes : PI 3 kinase p85/p55 (Phospho Tyr467/199) Antibody at 1/1k dilution

Lane 1 : COS7 cells treated with H2O2 100uM 30',using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is blocked with the phospho peptide.
Lysates/proteins at 20 ug per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution

Predicted band size: 83,54 kDa
Observed band size: 85,55 kDa

Exposure time: 15 seconds
Formalin-fixed, paraffin-embedded Human-colon tissue stained for PI 3 kinase p85/p55 (Phospho Tyr467/199) Antibody was diluted at 1:200 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence PI 3 kinase p85/p55 (Phospho Tyr467/199) Antibody

staining PI 3 kinase p85/p55 (Phospho Tyr467/199) Antibody in human-stomach tissue by ICC/IF.
Samples were incubated with Antibody at a working dilution of 1/200. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

If you have published an article using product 62171, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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