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ETBR Antibody#62213

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Product Detail

Product NameETBR Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB;IHC;IF

Species ReactivityHuman; Mouse; Rat

SpecificityThe antibody detects endogenous level of total ETBR protein.

Immunogen TypePeptide

Immunogen DescSynthesized peptide derived from ETBR at AA range: 31-80

Target NameETBR

ConjugateUnconjugated

Uniprot P24530

Gene ID 1910

Calculated MW50kDa

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details
IHC: 100-300; WB 1:500-2000; IF 1:50-200
All lanes : ETBR Antibody at 1/500 dilution
Lane 1 : Hela whole cell lysates
Lysates/proteins at 20 ug per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa

Exposure time: 3 seconds
Formalin-fixed, paraffin-embedded human-placenta tissue stained for ETBR at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded Human placenta tissue stained for ETBR at 1/200 dilution in immunohistochemical analysis.
The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016],

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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