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RhoA (Phospho Ser188) antibody#62231

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Product Detail

Product NameRhoA (Phospho Ser188) antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

ApplicationsWB, ELISA, IHC

Species ReactivityHuman, Mouse, Ra

SpecificityThis antibody detects endogenous levels of RhoA (Phospho Ser188).

Immunogen DescSynthesized peptide derived from human RhoA (Phospho Ser188).

Target NameRhoA

Uniprot P61586

Gene ID 387

Calculated MW22kD

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details
WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000
Western Blot analysis of mouse kidney, rat kidney, HuvEC with RhoA (Phospho Ser188) antibody at 1:1000. 1. mouse kidney whole cell lysate 2. rat kidney whole cell lysate 3. HuvEC whole cell lysate
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using RhoA (Phospho Ser188) antibody at 1:200.
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],

If you have published an article using product 62231, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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