Product Detail
Product NameUBE3B antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB, IHC, IF, ELISA
Species ReactivityHuman, Mouse
SpecificityUBE3B Polyclonal Antibody detects endogenous levels of UBE3B.
Immunogen DescThe antiserum was produced against synthesized peptide derived from human UBE3B. AA range:581-630
Target NameUBE3B
Uniprot
Q7Z3V4
Gene ID
89910
Calculated MW123kD
Concentration1mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.
Application Details
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200
Western blot analysis of lysates from Jurkat cells using UBE3B Antibody.
1. Jurkat whole cell lysate
2. Jurkat whole cell lysate blocked with the synthesized peptide
Immunohistochemical analysis of paraffin-embedded human tonsil using UBE3B Antibody at 1:200.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
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