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CD47 Mouse mAb#63397

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Product Detail

Product NameCD47 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG2a

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman

Immunogen DescPurified recombinant fragment of human CD47 (AA: 19-141) expressed in E. Coli.

Target NameCD47

Other NamesIAP; OA3; MER6

Accession NoQ08722

Uniprot Q08722

Gene ID 961

Calculated MW35.2kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using CD47 mouse mAb against Jurkat (1), MOLT4 (2), HL-60 (3), Raji (4), Ramos (5), and HEK293 (6) cell lysate.
Immunofluorescence analysis of Hela cells using CD47 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using CD47 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Jurkat cells using CD47 mouse mAb (green) and negative control (red).
Flow cytometric analysis of K562 cells using CD47 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using CD47 mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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