Product Detail
Product NameCD47 Mouse mAb
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG2a
ApplicationsWB;IHC;IF;FC
Species ReactivityHuman
Immunogen DescPurified recombinant fragment of human CD47 (AA: 19-141) expressed in E. Coli.
Target NameCD47
Other NamesIAP; OA3; MER6
Accession NoQ08722
Uniprot
Q08722
Gene ID
961
Calculated MW35.2kDa
FormulationPurified antibody in PBS with 0.05% sodium azide
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Application Details
WB:1/500 - 1/2000IHC:1/200 - 1/1000ICC:1/200 - 1/1000FC:1/200 - 1/400ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using CD47 mouse mAb against Jurkat (1), MOLT4 (2), HL-60 (3), Raji (4), Ramos (5), and HEK293 (6) cell lysate.
Immunofluorescence analysis of Hela cells using CD47 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using CD47 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Jurkat cells using CD47 mouse mAb (green) and negative control (red).
Flow cytometric analysis of K562 cells using CD47 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using CD47 mouse mAb with DAB staining.