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COL1A2 Mouse mAb#63494

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Product Detail

Product NameCOL1A2 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IF;FC

Species ReactivityHuman,Mouse,Rat,Monkey

Immunogen DescPurified recombinant fragment of human COL1A2 (AA: 23-79) expressed in E. Coli.

Target NameCOL1A2

Other NamesOI4; EDSCV; EDSARTH2

Accession NoP08123

Uniprot P08123

Gene ID 1278

Calculated MW129kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500-1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using THY1 mouse mAb against NIH/3T3 cell lysate.
Immunofluorescence analysis of Hela cells using COL1A2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of SW480 cells using COL1A2 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Hela cells using COL1A2 mouse mAb (green) and negative control (red).
Flow cytometric analysis of NIH/3T3 cells using COL1A2 mouse mAb (green) and negative control (red).
Flow cytometric analysis of COS7 cells using COL1A2 mouse mAb (green) and negative control (red).

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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