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Location： Home > Mouse Monoclonal Antibodies > COL2A1 Mouse mAb

COL2A1 Mouse mAb#63496

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Product Detail

Product NameCOL2A1 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IF;FC

Species ReactivityHuman

Immunogen DescPurified recombinant fragment of human COL2A1 (AA: 1222-1487) expressed in HEK293-6e cells supernatant.

Target NameCOL2A1

Other NamesAOM; ANFH; SEDC; STL1; COL11A3

Accession NoP02458

Uniprot P02458

Gene ID 1280

Calculated MW141.8kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
ICC:1/50 - 1/200
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using COL2A1 mouse mAb against Hela (1), MCF-7 (2),A549 (3),Jurkat (4), and K562 (5) cell lysate.
Immunofluorescence analysis of Hela cells using COL2A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Jurkat cells using COL2A1 mouse mAb (green) and negative control (red).

If you have published an article using product 63496, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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