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Location: Home > Mouse Monoclonal Antibodies > DDX20 Mouse mAb

DDX20 Mouse mAb#63538

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Product Detail

Product NameDDX20 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IF;FC

Species ReactivityHuman,Rat

Immunogen DescPurified recombinant fragment of human DDX20 (AA: 725-824) expressed in E. Coli.

Target NameDDX20

Other NamesDP103; GEMIN3

Accession NoQ9UHI6

Uniprot Q9UHI6

Gene ID 11218

Calculated MW92.2kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using DDX20 mouse mAb against Jurkat (1), Hela (2), NTERA-2 (3), HL7702 (4), K562 (5), and C6 (6) cell lysate.
Immunofluorescence analysis of HeLa cells using DDX20 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of HeLa cells using DDX20 mouse mAb (green) and negative control (red).

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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