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DDX3X Mouse mAb#63540

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Product Detail

Product NameDDX3X Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG2a

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Mouse,Rat,Monkey,Rabbit

Immunogen DescPurified recombinant fragment of human DDX3X (AA: 518-661) expressed in E. Coli.

Target NameDDX3X

Other NamesDBX; DDX3; HLP2; DDX14; CAP-Rf

Accession NoO00571

Uniprot O00571

Gene ID 1654

Calculated MW73.2kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/100 - 1/500
ICC:1/50 - 1/500
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.
Western blot analysis using DDX3X mouse mAb against PC-12(1) Raw264.7(2)NIH/3T3(3)NRK(4)C6(5)F9(6)COS-7(&)CHO3D10(8) cell lysate.
Immunofluorescence analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis of Hela cells using DDX3X mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using DDX3X mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using DDX3X mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse kidney using DDX3X mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat kidney using DDX3X mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit kidney using DDX3X mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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