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EIF4B Mouse mAb#63582

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Product Detail

Product NameEIF4B Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG2b

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Mouse

Immunogen DescPurified recombinant fragment of human EIF4B expressed in E. Coli.

Target NameEIF4B

Other NamesEIF-4B; PRO1843

Accession NoP23588

Uniprot P23588

Gene ID 1975

Calculated MW80kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.
Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using EIF4B mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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