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EIF4E Mouse mAb#63583

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Product Detail

Product NameEIF4E Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman

Immunogen DescPurified recombinant fragment of human EIF4E expressed in E. Coli.

Target NameEIF4E

Other NamesCBP; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E

Accession NoP06730

Uniprot P06730

Gene ID 1977

Calculated MW25kDa

FormulationAscitic fluid containing 0.03% sodium azide.

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western blot analysis using EIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.
Immunofluorescence analysis of GC-7901 cells using EIF4E mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of Hela cells using EIF4E mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human liver cancer (left) and submaxillary tumor (right) using EIF4E mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and prostate cancer (right) using EIF4E mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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