Product Detail
Product NameETFA Mouse mAb
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG2a
ApplicationsWB;IHC;IF;FC
Species ReactivityHuman
Immunogen DescPurified recombinant fragment of human ETFA (AA: 134-333) expressed in E. Coli.
Target NameETFA
Other NamesEMA; GA2; MADD
Accession NoP13804
Uniprot
P13804
Gene ID
2108
Calculated MW35kDa
FormulationPurified antibody in PBS with 0.05% sodium azide
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Application Details
WB:1/500 - 1/2000IHC:1/200 - 1/1000ICC:1/200 - 1/1000FC:1/200 - 1/400ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using ETFA mouse mAb against .HepG2 (1), A431 (2), Hek293 (3),Hela (4)and MCF-7 (5) cell lysate.
Immunofluorescence analysis of Hela cells using ETFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using ETFA mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using ETFA mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using ETFA mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using ETFA mouse mAb with DAB staining.