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HIF1A Mouse mAb#63725

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Product Detail

Product NameHIF1A Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;IF

Species ReactivityHuman,Mouse,Monkey

Immunogen DescPurified recombinant fragment of human HIF1A expressed in E. Coli.

Target NameHIF1A

Other NamesHIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF1A

Accession NoQ16665

Uniprot Q16665

Gene ID 3091

Calculated MW120kDa

FormulationPurified antibody in PBS with 0.05% sodium azide.

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
ELISA:1/10000
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western blot analysis using HIF1A mouse mAb against Cos7 (1), Hela (2), Jurkat (3), RAJI (4) and NIH/3T3 (5) cell lysate.
Immunofluorescence analysis of Hela cells using HIF1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (left) and lung cancer tissues (right) using HIF1A mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues (left) and brain tumor tissues (right) using HIF1A mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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