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HIF2a Mouse mAb#63726

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Product Detail

Product NameHIF2a Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsIHC;IF;FC

Species ReactivityHuman

Immunogen DescPurified recombinant fragment of human HIF2a (AA: 680-870) expressed in E. Coli.

Target NameHIF2a

Other NamesHLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73

Accession NoQ99814

Uniprot Q99814

Gene ID 2034

Calculated MW96.5kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Immunofluorescence analysis of Hela cells using HIF2a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using HIF2a mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using HIF2a mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human baldder cancer tissues using HIF2a mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human kidney tissues using HIF2a mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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