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Location: Home > Mouse Monoclonal Antibodies > HSP70 Mouse mAb

HSP70 Mouse mAb#63751

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Product Detail

Product NameHSP70 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Monkey

Immunogen DescPurified recombinant fragment of human HSP70 (AA: 642-841) expressed in mammalian.

Target NameHSP70

Other NamesRY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52

Accession NoP34932

Uniprot P34932

Gene ID 3308

Calculated MW94.3kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using HSP70 mouse mAb against Hela (1), HepG2 (2),Hek293 (3),COS-7 (4),A549 (5) and Jurkat (6) cell lysate.
Immunofluorescence analysis of Hela cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red).
Flow cytometric analysis of K562 cells using HSP70 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using HSP70 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using HSP70 mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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