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HSPA9 Mouse mAb#63753

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Product Detail

Product NameHSPA9 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Rat,Monkey

Immunogen DescPurified recombinant fragment of human HSPA9 (AA: 480-679) expressed in mammalian.

Target NameHSPA9

Other NamesCSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; SIDBA4; MTHSP75; HEL-S-124m

Accession NoP38646

Uniprot P38646

Gene ID 3313

Calculated MW74KDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using HSPA9 mouse mAb against CSO-7 (1),C6 (2), PC-12 (3), PANC-1(4),A549 (5),MCF-7 (6),K562 (7),Hela (8),A431 (9),HepG2 (10)and Jurkat (11) cell lysate.
Immunofluorescence analysis of Hela cells using HSPA9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using HSPA9 mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using HSPA9 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Jurkat cells using HSPA9 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using HSPA9 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using HSPA9 mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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