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IL1B Mouse mAb#63779

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Product Detail

Product NameIL1B Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsIHC;IF;FC

Species ReactivityHuman

Immunogen DescPurified recombinant fragment of human IL1B (AA: 117-269) expressed in E. Coli.

Target NameIL1B

Other NamesIL-1; IL1F2; IL1beta; IL1-BETA

Gene ID 3553

Calculated MW30.7kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Immunofluorescence analysis of Hela cells using IL1B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Jurkat cells using IL1B mouse mAb (green) and negative control (red).
Flow cytometric analysis of MOLT4 cells using IL1B mouse mAb (green) and negative control (red).
Flow cytometric analysis of THP-1 cells using IL1B mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using IL1B mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using IL1B mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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