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MYLK Mouse mAb#63977

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Product Detail

Product NameMYLK Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Mouse

Immunogen DescPurified recombinant fragment of human MYLK (AA:1375-1524) expressed in E. Coli.

Target NameMYLK

Other NamesKRP; AAT7; MLCK; MLCK1; MMIHS; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083

Accession NoQ15746

Uniprot Q15746

Gene ID 4638

Calculated MW210kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200-1/1000
ICC:1/200 - 1/1000
FC:1/200-1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Western blot analysis using MYLK mouse mAb against PC-3 (1), C2C12 (2), and Hela (3) cell lysate.
Immunofluorescence analysis of Hela cells using MYLK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of hela cells using MYLK mouse mAb (green) and negative control (red).
Flow cytometric analysis of LNCAP cells using MYLK mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder Cancer tissues using MYLK mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissues using MYLK mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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