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NEDD9 Mouse mAb#63991

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Product Detail

Product NameNEDD9 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IF;FC

Species ReactivityHuman,Rat

Immunogen DescPurified recombinant fragment of human NEDD9 (AA: 82-398) expressed in E. Coli.

Target NameNEDD9

Other NamesCAS2; CASL; HEF1; CAS-L; CASS2

Accession NoQ14511

Uniprot Q14511

Gene ID 4739

Calculated MW92.9kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using NEDD9 mouse mAb against MCF-7 (1), Hela (2), C2C12 (3),and Hek293 (4) cell lysate.
Immunofluorescence analysis of Hela cells using NEDD9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of HepG2 cells using NEDD9 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Jurkat cells using NEDD9 mouse mAb (green) and negative control (red).
Flow cytometric analysis of K562 cells using NEDD9 mouse mAb (green) and negative control (red).

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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