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p44/42 MAPK (Erk1/2) Mouse mAb#64046

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Product Detail

Product Namep44/42 MAPK (Erk1/2) Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG2b

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Mouse,Rabbit

Immunogen DescPurified recombinant fragment of human MAPK expressed in E. Coli.

Target Namep44/42 MAPK (Erk1/2)

Other NamesERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; MAPK1

Accession NoP28482

Uniprot P28482

Gene ID 5594

Calculated MW42 , 44kDa

FormulationAscitic fluid containing 0.03% sodium azide.

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/100 - 1/500
ICC:1/50 - 1/500
FC:1/200 - 1/400
ELISA:1/10000
Western blot analysis using p44/42 MAPK mouse mAb against Jurkat (1), Hela (2), A431 (3) and NIH/3T3 (4) cell lysate.
Western blot analysis using p44/42 MAPK (Erk1/2) mouse mAb against Rat liver(1)mouse liver (2) PC-12(3) Raw264.7 (4) NIH/3T3(5) NRK(6) C2C12(7) C6(8) L1210(9) F9 (10) COS-7 (11) CHO3D10(12) cell lysate.
Immunofluorescence analysis of COS7 cells using p44/42 MAPK (Erk1/2) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis of NIH/3T3 cells using p44/42 MAPK (Erk1/2) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis of PC-12 cells using p44/42 MAPK (Erk1/2) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow cytometric analysis of Jurkat cells using p44/42 MAPK mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human Liver tissues using anti p44/42 MAPK (Erk1/2) mouse mAb
Immunohistochemical analysis of paraffin-embedded Mouse brain(A)Mouse kidney(B) using p44/42 MAPK (Erk1/2) mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat liver(A)Rat kidney(B) using p44/42 MAPK (Erk1/2) mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit liver(A)Rabbit kidney(B) using p44/42 MAPK (Erk1/2) mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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