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P4HB Mouse mAb#64047

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Product Detail

Product NameP4HB Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG2a

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman

Immunogen DescPurified recombinant fragment of human P4HB (AA:309-508) expressed in mammalian.

Target NameP4HB

Other NamesDSI,GIT, PDI,PHDB, PDIA1,PO4DB, PO4HB,PROHB,CLCRP1, ERBA2L,P4Hbeta

Accession NoP07237

Uniprot P07237

Gene ID 5034

Calculated MW57.1kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using P4HB mouse mAb against Hela (1), PANC-1 (2), MCF-7 (3),THP-1 (4),SW620 (5)and HepG2 (6)cell lysate.
Immunofluorescence analysis of Hela cells using P4HB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using P4HB mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using P4HB mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using P4HB mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using P4HB mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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