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PDGFA Mouse mAb#64063

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Product Detail

Product NamePDGFA Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG2a

ApplicationsIF;FC

Species ReactivityHuman,Mouse

Immunogen DescPurified recombinant fragment of human PDGFA (AA: 87-211) expressed in E. Coli.

Target NamePDGFA

Other NamesPDGF1; PDGF-A

Accession NoP04085

Uniprot P04085

Gene ID 5154

Calculated MW24kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
ICC:1/50 - 1/200
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Immunofluorescence analysis of Hela cells using PDGFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis of NIH/3T3 cells using PDGFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Jurkat cells using PDGFA mouse mAb (green) and negative control (red).

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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