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PLK1 Mouse mAb#64100

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Product Detail

Product NamePLK1 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;FC

Species ReactivityHuman,Mouse,Rat,Monkey

Immunogen DescPurified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli.

Target NamePLK1

Other NamesPLK;STPK13

Accession NoP53350

Uniprot P53350

Gene ID 5347

Calculated MW68kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/100 - 1/500
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using PLK1 mouse mAb against K562 (1) and Raji (2) cell lysate.
Western blot analysis using PLK1 mouse mAb against NIH/3T3(1)NRK(2)C6(3)COS-7(4)CHO3D10(5) cell lysate.
Flow cytometric analysis of NIH3T3 cells using PLK1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PLK1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using PLK1 mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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